Answered

Phosphate buffer solution is purchased as a(n) 10x stock solution. The solution is more concentrated than the working stock. If tasked with making 941 mL of a 1x solution of PBS, how many mL of the stock solution would be needed

Answer :

94.1 mL of 10x PBS should be needed

Given:

Phosphate buffer solution = 10x

Volume of 1x solution of PBS = 941 mL

To Find:

Volume of stock solution

Solution:

A buffer solution is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa. Its pH changes very little when a small amount of strong acid or base is added to it.

using the formula

M1 x V1 = M2 x V2

(10x)* V1 = 1x* 941

V1 = 94.1 ml

So, 94.1 mL of 10x PBS should be needed

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