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Organisms share many conserved core processes and features, including transcription and translation, using a uniform genetic code. Scientists have used these shared processes and features in biotechnology. For example, for the process of some transformations, a plasmid is constructed when a eukaryotic gene of interest is added with an antibiotic resistant gene such as beta- lactamase, which is used for ampicillin resistance. This plasmid is then inserted into a prokaryotic bacterial cell, such as E. coli, through a transformation process that leads to the production of the product protein from the eukaryotic organism. To culture the bacteria and obtain the protein product, the bacteria must grow. Select the appropriate condition to determine if the plasmid has entered the E. coli bacterial cell.

Answer :

Using an ampicillin-infused nutrient broth is the proper method for determining whether plasmid has entered the E. coli bacterial cell.

What exactly is antibiotic resistance?

Antibiotics, antivirals, antifungals, and antiparasitics are just a few examples of the medications and other substances referred to as "antimicrobials" that are used to treat and prevent infections.

Antimicrobial Resistance, or AMR, is a defense mechanism created by bacteria, viruses, fungi, and parasites when they alter their structure or function and make antimicrobials useless to them.

This enhances the transmission of infectious diseases and makes it harder to treat the infections they produce. The correct option is C.

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Question:-

Use the following information to answer the question.

Organisms share many conserved core processes and features, including transcription and translation, using a uniform genetic code. Scientists have used these shared processes and features in biotechnology. For example, for the process of some transformations, a plasmid is constructed when a eukaryotic gene of interest is added with an antibiotic resistant gene such as beta-lactamase, which is used for ampicillin resistance. This plasmid is then inserted into a prokaryotic bacterial cell, such as E . coli, through a transformation process that leads to the production of the product protein from the eukaryotic organism. To culture the bacteria and obtain the protein product, the bacteria must grow.

Select the appropriate condition to determine if the plasmid has entered the E coli bacterial cell. a. nutrient broth to which no antibiotic has been added b. water to which ampicillin has been added c. nutrient broth to which ampicillin has been added d. nutrient broth to which other resistant bacteria have been added

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